- Pds1 / Esp1 Mutants
- pds1∆
- pds1∆ in nocodazole
- bub2∆ pds1∆ in nocodazole
- mad2∆ pds1∆ in nocodazole
- cdc20∆ pds1∆
- cdc20∆ pds1∆ clb5∆
- CLB5-db∆ pds1∆
- CLB5-db∆ pds1∆ cdc20∆
- CLB1 clb2∆ pds1∆
- esp1-ts
- GAL-PDS1-db∆ esp1-ts
- GAL-PDS1-db∆
- PDS1-db∆
- GAL-ESP1 cdc20-ts
cdc20∆ pds1∆
debug: ,
test user =
test db =
Simulation:
Change of parameters: ks20'=ks20"=0, ks1pds"=ks2pds"=0.
Length of G1 phase: 35.3 min.
Arrest: Telophase arrest.
Experiments:
Lim, H.H., Goh, P.-Y. and Surana, U. (1998). Cdc20 is essential for the cyclosome-mediated proteolysis of both Pds1 and Clb2 during M phase in budding yeast. Curr. Biol. 8:231-234.
[Abstract] [Article]
[Abstract] [Article]
Shirayama, M., Zachariae, W., Ciosk, R. and Nasmyth, K. (1998). The Polo-like kinase Cdc5p and the WD-repeat protein Cdc20p/fizzy are regulators and substrates of the anaphase promoting complex in Saccharomyces cerevisiae. EMBO J. 17:1336-1349.
[Abstract] [Article]
[Abstract] [Article]
Shirayama, M., Toth, A., Galova, M. and Nasmyth, K. (1999). APC(CDC20) promotes exit from mitosis by destroying the anaphase inhibitor Pds1 and cyclin Clb5. Nature 402:203-207.
[Abstract] [Article]
[Abstract] [Article]
Experimental results: Lim, Fig. 8 and Shirayama, Fig. 8, inviable; Shirayama, Fig. 1, telophase arrest.
Comments:
webmaster